Underhill and Pan publish Nitro-PTS work

Former postdoc Dr. Simon Underhill and PhD candidate Somalisa Pan published a big project in which they examined the nitrogen-related phosphotransfer system (Nitro-PTS) in P. aeruginosa in unprecedented detail. Mary Erdmann, a talented undergraduate working with Soma, was also a coauthor. The team showed that unphosphorylated PtsN, which was previously implicated in lowering biofilm formation, is not sufficient on its own to reduce biofilm formation. They also showed that PtsN can be phosphorylated by a different enzyme I, FruB, but only in the absence of PtsO. PtsP also appears to be able to directly phosphorylate PtsN. Finally, using transcriptomics, they discover new systems that appear to be regulated by the Nitro-PTS, including production of a siderophore (pyoverdine) and express of Type III secretion. Congratulations Simon, Soma, and Mary! The paper is available in the Journal of Bacteriology at https://doi.org/10.1128/jb.00453-22 (may require a journal subscription).